This ELISA kit can be applied to the in vitro quantitative determination of Cortisol concentrations in serum, plasma.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Cortisol. During the reaction, Cortisol in the sample or standard competes with a fixed amount of Cortisol on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Cortisol. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay type: Competitive
Assay time 2.0h
Detection Method Colormetric
Detection Range 1.56—100 ng/mL
Sensitivity 0.96 ng/mL
Sample Volume 50μL
Sample Type serum, plasma
This kit recognizes Cortisol in samples. No significant cross-reactivity or interference between Cortisol and analogues was observed.
Other supplies required
Microplate reader with 450 nm wavelength filter
High-precision transfer pipette, EP tubes and disposable pipette tips
Incubator capable of maintaining 37°C
Deionized or distilled water
Loading slot for Wash Buffer
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