SARS-CoV-2 Neutralization Antibody ELISA Kit, 96T
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Cat.No.:E-EL-E606
- Reactivity: Human
- Assay Type: One step Competitive-ELISA
- Assay time: 1.5h
Properties
| Format | 24T, 96T, 96T*5, 96T*10 |
| Assay time | 1.5h |
| Sample type | serum and plasma |
| Sample volume | 50 μL |
| Storage | 2-8℃ |
| Interpretation | Qualitative |
| Application | This ELISA kit applies to the in vitro Qualitative determination of SARS-CoV-2 Neutralization Antibody in serum or plasma. |
| Reproducibility | Both intra-CV and inter-CV are < 15% |
| Synonyms | COVID 19 Neutralization Antibody, Anti-RBD, RBD Antibody, S1RBD Antibody |
Test Principle
This ELISA kit uses Competitive-ELISA as the method to qualitatively detect the Anti-SARS-CoV-2 Neutralization Antibody in the sample. _x000D_ The micro ELISA plate provided in this kit is pre-coated with recombinant human ACE2. During the reaction, the SARS-CoV-2 Neutralization Antibody in the pretreated samples or controls competes with a fixed amount of human ACE2 on the solid phase supporter for sites on the Horseradish peroxidase (HRP) conjugated recombinant SARS-CoV-2 RBD fragment (HRP-RBD). After 37℃ incubation, the unbound HRP-RBD as well as any HRP-RBD bound to non-Neutralization antibody will be captured on the plate and eventually form the ACE2-RBD-HRP complex, while the circulating neutralization antibodies HRP-RBD complexes remain in the supernatant and are removed during washing. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. Compared with the inhibition ratio to judge whether SARS-CoV-2 Neutralization Antibody exists in the tested samples or not.
Assay Procedures
Conteúdo
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