SARS-CoV-2 Spike Protein IgM ELISA Kit, 96T
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Cat.No.:E-EL-E603
- Reactivity: Human
- Assay Type: Indirect-ELISA
- Assay time: 1.5h
Properties
| Format | 24T, 96T, 96T*5, 96T*10 |
| Assay time | 1.5h |
| Sample type | serum and plasma |
| Sample volume | 100μL |
| Storage | 2-8℃ |
| Interpretation | Qualitative |
| Application | This ELISA kit applies to the in vitro Qualitative determination of SARS-CoV-2 IgM in serum or plasma. |
| Reproducibility | Both intra-CV and inter-CV are < 10% |
| Synonyms | COVID 19 IgM,S protein IgM |
Test Principle
This ELISA kit uses Indirect-ELISA as the method to qualitatively detect the SARS-CoV-2 Spike protein IgM in the sample. The micro ELISA plate provided in this kit is pre-coated with purified SARS-CoV-2 Spike protein (PKSR030489) antigen, after adding samples to wells, the SARS-CoV-2 Spike protein IgM in the samples will combine with the pre-coated SARS-CoV-2 Spike protein antigen. After washing completely, add Horseradish Peroxidase (HRP) conjugated mouse anti human IgM to develop the antigen-antibody-HRP conjugated secondary antibody complex. Free components are washed away, then the substrate solution is added to each well. Only those wells that contain SARS-CoV-2 Spike protein IgM and HRP conjugated anti-human IgM will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Spike protein IgM exists in the tested samples or not.
Assay Procedures
(RUO) FOR RESEARCH USE ONLY. PARA USO EXCLUSIVO EM PESQUISA CIENTIFICA.
Conteúdo
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A ABKIT é uma empresa especializada no fornecimento de anticorpos, kits ELISA, proteínas e equipamentos para laboratório. Nossa linha abrange uma lista completa de produtos para diversas áreas de pesquisa científica incluindo imunologia, neurociência, câncer, metabolismo e biologia celular.
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